Recent publications of project members regarding foodborne and waterborne enteric viruses, AMR and SARS-CoV-2:

Perry, W.B., Chrispim, M.C, Funada Barbosa, M.R., de Souza Lauretto, M., Razzolini, M., C. Nardocci, A.C., Jones, O., Jones, D.L., Weightman, A.J., Zanoli Sato, M.I., Montagner, C., Durance, I., 2024. Cross-continental comparative experiences of wastewater surveillance and a vision for the 21st Century. Science of the Total Environment in press.

Jameson, E., Ellison, A (2024) One Health Comic on Wastewater Based Epidemiology. 

Leoni Kevill, J., Farkas, K., Ridding, N., Woodhall, N., Malham, S.K., Jones, D.L., 2024. Use of Viability-qPCR for Assessing Viral Capsid Integrity in Wastewater. Viruses 16, 40.

Nan, X., Hardinge, P., Hoehn, S. et al. (2023) VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. Scientific Reports 13, 20832.

Jones, D.L. (2023) House of Commons, Welsh Affairs Committee, Oral evidence: Water Quality in Wales, HC 104.

Farkas, K., Pântea, I., Woodhall, N., Williamsn, D., Lambert-Slosarska, K., Williams, R.C., Grimsley, J.M.S., Singer, A.C., Jones, D.L., 2023. Diurnal changes in pathogenic and indicator virus concentrations in wastewater. Environ Sci Pollut Res (2023).

Alex-Sanders, N., Woodhall, N., Farkas, K., Scott, G., Jones, D.L., Walker, D.I., (2023) Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples.Journal of Virological Methods 321, 114804.

Kathryn Lambert-Slosarska (2023) Use of passive samplers for the capture of SARS-CoV-2 and other viruses from wastewater. Masters thesis, Bangor University.

Keshaviah, A., Diamond, M.B., Wade, M.J., Scarpino, S.V. and the Global Wastewater Action Group involving Jones, D.L. (2023) Wastewater monitoring can anchor global disease surveillance systems. Lancet Global Health 11, 976-981.

Chigor, V. N., Chidebelu, P. E., Digwo, D. C., Chigor, C. B., Nwagwu, A. U., Udeh, O. S., Oguonu, C. I., Dibua, M.-E. U., & Farkas, K. (2023). Assessment of the aetiology of acute gastroenteritis outbreaks in infants reveals rotavirus, noroviruses and adenovirus prevalence and viral coinfections in Nsukka, Nigeria. VirusDisease.

Jones, D. L., Rhymes, J. M., Green, E., Rimmer, C., Kevill, J. L., Malham, S. K., Weightman, A. J., & Farkas, K. (2023). Poor Air passenger knowledge of COVID-19 symptoms and behaviour undermines strategies aimed at preventing the import of SARS-COV-2 into the UK. Scientific Reports, 13(1).

Kasprzyk-Hordern, B., Sims, N., Farkas, K., Jagadeesan, K., Proctor, K., Wade, M. J., & Jones, D. L. (2023). Wastewater-based epidemiology for Comprehensive Community Health Diagnostics in a national surveillance study: Mining biochemical markers in wastewater. Journal of Hazardous Materials, 130989.

Welsh Government (2023) Example of our Wastewater SARS-CoV-2 Variants of Concern weekly report.

Farkas, K., Williams, R., Alex-Sanders, N., Grimsley, J. M., Pântea, I., Wade, M. J., Woodhall, N., & Jones, D. L. (2023). Wastewater-based monitoring of SARS-COV-2 at UK airports and its potential role in international public health surveillance. PLOS Global Public Health, 3(1).

Robins, P.E., Dickson, N., Kevill, J.L., Malham, S.K., Singer, A.C., Quilliam, R.S., Jones, D.L. (2022) Predicting the dispersal of SARS-CoV-2 RNA from the wastewater treatment plant to the coast. Helyion 8, e10547. DOI:10.1016/j.heliyon.2022.e10547

Farkas, K., Pellett, C., Williams, R., Alex-Sanders, N., Bassano, I., Brown, M. R., Denise, H., Grimsley, J. M., Kevill, J. L., Khalifa, M. S., Pântea, I., Story, R., Wade, M. J., Woodhall, N., & Jones, D. L. (2023). Rapid assessment of SARS-COV-2 variant-associated mutations in wastewater using real-time RT-PCR. Microbiology Spectrum.

Wilde, H., Perry, W.B., Jones, O., Kille, P., Weightman, A., Jones, D.L., Cross, G., Durance, I. (2022) Accounting for Dilution of SARS-CoV-2 in Wastewater Samples Using Physico-Chemical Markers. Water 14, 2885. DOI:10.3390/w14182885

Brunner, F. S., Brown, M. R., Bassano, I., Denise, H., Khalifa, M. S., Wade, M. J., van Aerle, R., Kevill, J. L., Jones, D. L., Farkas, K., Jeffries, A. R., Cairns, E., Wierzbicki, C., & Paterson, S. (2022). City-wide wastewater genomic surveillance through the successive emergence of SARS-COV-2 alpha and Delta variants. Water Research, 226, 119306.

Jones, D. L., Grimsley, J. M., Kevill, J. L., Williams, R., Pellett, C., Lambert-Slosarska, K., Singer, A. C., Williams, G. B., Bargiela, R., Brown, R. W., Wade, M. J., & Farkas, K. (2022). Critical evaluation of different passive sampler materials and approaches for the recovery of SARS-COV-2, faecal-indicator viruses and bacteria from wastewater. Water, 14(21), 3568.

Jones, D. L., Rhymes, J. M., Wade, M. J., Kevill, J. L., Malham, S. K., Grimsley, J. M. S., Rimmer, C., Weightman, A. J., & Farkas, K. (2023). Suitability of aircraft wastewater for Pathogen Detection and Public Health Surveillance. Science of The Total Environment, 856, 159162.

Robins, K., Leonard, A. F., Farkas, K., Graham, D. W., Jones, D. L., Kasprzyk-Hordern, B., Bunce, J. T., Grimsley, J. M., Wade, M. J., Zealand, A. M., & McIntyre-Nolan, S. (2022). Research needs for optimising wastewater-based epidemiology monitoring for Public Health Protection. Journal of Water and Health, 20(9), 1284–1313.

Farkas, K., Pellett, C., Alex-Sanders, N., Bridgman, M. T., Corbishley, A., Grimsley, J. M., Kasprzyk-Hordern, B., Kevill, J. L., Pântea, I., Richardson-O’Neill, I. S., Lambert-Slosarska, K., Woodhall, N., & Jones, D. L. (2022). Comparative assessment of filtration- and precipitation-based methods for the concentration of SARS-COV-2 and other viruses from wastewater. Microbiology Spectrum, 10(4).

Kevill, J. L., Lambert-Slosarska, K., Pellett, C., Woodhall, N., Richardson-O’Neill, I., Pântea, I., Alex-Sanders, N., Farkas, K., & Jones, D. L. (2022). Assessment of two types of passive sampler for the efficient recovery of SARS-COV-2 and other viruses from wastewater. Science of The Total Environment, 838, 156580.

Wastewater Monitoring in Wales, National COVID-19 Report (released every week). Example: Report Date: 17 March 2022. Welsh Government.

Tlhagale, M., Liphadzi, S., … Jones, D.L. (2022) Establishment of local wastewater-based surveillance programmes in response to the spread and infection of COVID-19-case studies from South Africa, the Netherlands, Turkey and England. Journal or Water and Health 20, 287–299.

Adriaenssens, E. M., Farkas, K., McDonald, J. E., Jones, D. L., Allison, H. E., & McCarthy, A. J. (2021). Tracing the fate of wastewater viruses reveals catchment-scale virome diversity and connectivity. Water research203, 117568.

Fitzgerald, S. F., Rossi, G., Low, A. S., McAteer, S. P., O’Keefe, B., Findlay, D., Cameron, G. J., Pollard, P., Singleton, P., Ponton, G., Singer, A. C., Farkas, K., Jones, D., Graham, D. W., Quintela-Baluja, M., Tait-Burkard, C., Gally, D. L., Kao, R., & Corbishley, A. (2021). Site Specific Relationships between COVID-19 Cases and SARS-CoV-2 Viral Load in Wastewater Treatment Plant Influent. Environmental Science & Technology55(22), 15276–15286.

Singer, A.C., Jones, D.L. (2022) Testing sewage has helped track COVID – soon it could reveal much more about the UK’s health. The Conversation 23-03-2022

Kevill, J., Pellett, C., Farkas, K., Brown, M., Bassano, I., Denise, H., McDonald, J., Malham, S., Porter, J., Warren, J., Evens, N., Paterson, S., Singer, A. and Jones, D. (2022) A comparison of precipitation and filtration-based SARS-CoV-2 recovery methods and the influence of temperature, turbidity, and surfactant load in urban wastewater. Science of The Total Environment, 808: 151916.

Wade, M., Lo Jacomo, A., Armenise, E., Brown, M., Bunce, J., Cameron, G., Fang, Z., Farkas, K., Gilpin, D., Graham, D., Grimsley, J., Hart, A., Hoffmann, T., Jackson, K., Jones, D., Lilley, C., McGrath, J., McKinley, J., McSparron, C., Nejad, B., Morvan, M., Quintela-Baluja, M., Roberts, A., Singer, A., Souque, C., Speight, V., Sweetapple, C., Walker, D., Watts, G., Weightman, A. and Kasprzyk-Hordern, B. (2021) Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes. Journal of Hazardous Materials 424:127456.

Welsh Government COVID-19 Technical Advisory Group (2021) Mitigating risks of increased COVID-19 transmission during elections.

Welsh Government COVID-19 Technical Advisory Group (2021) SARS-CoV-2 transmission risk in public toilets.

Welsh Government COVID-19 Technical Advisory Group (2021) COVID-19 evidence associated with transmission and potential risks associated with religious activities and places of worship.

Welsh Government COVID-19 Technical Advisory Group (2021) review advice on communal singing and chanting, including wind instruments and pipe organs.

Welsh Government COVID-19 Technical Advisory Group (2021) plastic screens in taxis and private hire vehicles.

Welsh Government COVID-19 Technical Advisory Group (2021) use of face coverings in childcare and educational settings for Under 18s.

Welsh Government COVID-19 Technical Advisory Group (2021) advice for nightclubs and adult entertainment venues.

Welsh Government COVID-19 Technical Advisory Group (2021) evidence review of ozone generators including appropriateness as mitigation in classrooms.

Welsh Government COVID-19 Technical Advisory Group (2022) reducing COVID-19 transmission in schools and colleges: the role of air cleaning devices amongst other mitigations.

Welsh Government COVID-19 Technical Advisory Group (2022) Consensus statement on face masks for the public.

Welsh Government COVID-19 Technical Advisory Group (2022) Wastewater monitoring: 19 May 2022.

Dancer, S., Li, Y., Hart, A., Tang, J., Jones, D.L. (2021) What is the risk of acquiring SARS-CoV-2 from the use of public toilets? The Science of the Total Environment 792:148341.

Farkas, K., Green, E., Rigby, D. Cross, P., Tyrrel, S., Malham, S.K., Jones, D.L. (2021) Investigating awareness, fear and control associated with norovirus and other pathogens and pollutants using best–worst scaling. Scientific Reports 11,11194.

Hillary, L. S., Farkas, K., Maher, K. H., Lucaci, A., Thorpe, J., Distaso, M. A., Gaze, W. H., Paterson, S., Burke, T., Connor, T. R., McDonald, J. E., Malham, S. K., & Jones, D. L. (2021). Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK. Water Research, 117214.

Bashawri, YM (2021) Impact of Sediment Concentration on the Survival of Wastewater-Derived bla(CTX-M-15)-Producing E. coli, and the Implications for Dispersal into Estuarine Waters. International Journal of Environmental Research and Public Health 17, 7608.

Polo, D., et al. (2020) Making waves: Wastewater-based epidemiology for COVID-19-approaches and challenges for surveillance and prediction. Water Research 186, 116404.

Farkas, K., Hillary, LS., Thorpe, J., Walker, DI., Lowther, JA., McDonald, JE., Malham, SK, Jones DL  (2021) Concentration and Quantification of SARS-CoV-2 RNA in Wastewater Using Polyethylene Glycol-Based Concentration and qRT-PCR. Methods and Protocols 4, 17.

Wade, M., et al. (2020) Wastewater COVID-19 monitoring in the UK: summary for SAGE – 19 November 2020.

Jones, D.L., Baluja, M.Q., Graham, D.W., et al. (2020) Shedding of SARS-CoV-2 in feces and urine and its potential role in person-to-person transmission and the environment-based spread of COVID-19. Science of the Total Environment 749, 141364. 

Ahmed, W., Bertsch, P., Bivins, A., Bibby, K., Farkas, K., Gathercole, A., … & Mueller, J. (2020). Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater. Science of the Total Environment, 739, 139960.

Farkas, K., Hillary, L. S., Malham, S. K., McDonald, J. E., & Jones, D. L. (2020). Wastewater and public health: the potential of wastewater surveillance for monitoring COVID-19. Current Opinion in Environmental Science & Health. 17, 14-20.

Bivins, A., North, D., Ahmad, A., Ahmed, W., Alm, E., Been, F., … & Buttiglieri, G. (2020). Wastewater-Based Epidemiology: Global Collaborative to Maximize Contributions in the Fight Against COVID-19. Environmental Science and Technology,

Farkas, K., Walker, D. I., Adriaenssens, E. M., McDonald, J. E., Hillary, L. S., Malham, S. K., & Jones, D. L. (2020). Viral indicators for tracking domestic wastewater contamination in the aquatic environment. Water Research, 181, 115926.

UK Government TWEG (2020) Environmental monitoring of viral presence, infectivity and transmission of SARS-CoV-2

UK Government EMG/SPI-B/TWEG (2020) Mitigations to reduce transmission of the new variant SARS-CoV-2 virus, 22 December 2020

UK Government TWEG (2020) Evidence of wider environmental transmission of SARS-CoV-2, 12 June 2020

Welsh Government COVID-19 Technical Advisory Group (2020) swimming pools, hot tubs, saunas and steam rooms and risk from COVID-19.

Welsh Government COVID-19 Technical Advisory Group (2020) SARS-CoV-2 infection risks at ice rinks.

Welsh Government COVID-19 Technical Advisory Group (2020) SARS-CoV-2 infection risks at indoor exercise facilities.

Welsh Government COVID-19 Technical Advisory Group (2020) current evidence relating to weddings.

Welsh Government COVID-19 Technical Advisory Group (2020) air cleaning devices.

Welsh Government COVID-19 Technical Advisory Group (2020) Surveillance approaches to determine the risk, frequency and origin of SARS-CoV-2 entry into UK airports.

Farkas, K., Mannion, F., Hillary, L. S., Malham, S. K., & Walker, D. I. (2020). Emerging technologies for the rapid detection of enteric viruses in the aquatic environment. Current Opinion in Environmental Science & Health. 16, 1-6.

Robins, P. E., Farkas, K., Cooper, D., Malham, S. K., & Jones, D. L. (2019). Viral dispersal in the coastal zone: A method to quantify water quality risk. Environment International, 126, 430-442.

Farkas, K., Adriaenssens, E.M., Walker, D.I. et al. (2019) Critical Evaluation of CrAssphage as a Molecular Marker for Human-Derived Wastewater Contamination in the Aquatic Environment. Food and Environmental Virology. 11, 113-119.

Lowther, J. A., Cross, L., Stapleton, T., Gustar, N. E., Walker, D. I., Sills, M., … & Lees, D. N. (2019). Use of F-Specific RNA Bacteriophage to Estimate Infectious Norovirus Levels in Oysters. Food and Environmental Virology, 11(3), 247-258.

Walker, D. I., Cross, L. J., Stapleton, T. A., Jenkins, C. L., Lees, D. N., & Lowther, J. A. (2019). Assessment of the Applicability of Capsid-Integrity Assays for Detecting Infectious Norovirus Inactivated by Heat or UV Irradiation. Food and environmental virology, 11(3), 229-237.

Farkas, K., Marshall, M., Cooper, D., McDonald, J., Malham, S., Peters, D., Maloney, J., Jones, D. (2018) Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric viruses. Environmental Science and Pollution Research

Farkas, K., McDonald, J.E., Malham, S.K., Jones, D.L. (2018) Two-Step Concentration of Complex Water Samples for the Detection of Viruses. Methods and Protocols. 1, 35.

Adriaenssens, E., Farkas, K., Harrison, C., Jones, D.L., Allison, H.E., McCarthy, A.J. (2018) Viromic analysis of wastewater input to a river catchment reveals a diverse assemblage of RNA viruses. mSystems.

Farkas, K., Cooper, D.M., McDonald, J.E., Malham, S. K., de Rougemont, A., Jones, D. (2018) Seasonal and spatial dynamics of enteric viruses in wastewater and in riverine and estuarine receiving waters. Science of the Total Environment, 634:1174-1183

Farkas, K., Peters, D.E., McDonald, J.E., de Rougemont, A., Malham, S. K., Jones, D. (2017) Evaluation of Two Triplex One-Step qRT-PCR Assays for the Quantification of Human Enteric Viruses in Environmental Samples. Food and Environmental Virology, 9(3):342–349.

Hassard, F., Sharp, J. H., Taft, H., Le Vay, L., Harris, J. P., McDonald, J. E., Tuson, K., Wilson, J., Jones, D., Malham, S. (2017) Critical Review on the Public Health Impact of Norovirus Contamination in Shellfish and the Environment: A UK Perspective. Food and Environmental Virology, 9(2):123–141.

Farkas, K., Hassard, F., McDonald, J. E., Malham, S. K. and Jones, D. (2016) Evaluation of molecular methods for the detection and quantification of pathogen-derived nucleic acids in sediment.  Frontiers in Microbiology.

Hassard, F., Gwyther, C. L.,  Farkas, K.,  Andrews, A.,  Jones, V., Cox, B.,  Brett, H., Jones, D.,  McDonald, J. E., and  Malham, S. K.  (2016)   Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review.  Frontiers in Microbiology.

Campos, C. J., Kershaw, S., Morgan, O.C. & Lees, D. N. (2016). Risk factors for norovirus contamination of shellfish water catchments in England and Wales. International Journal of Food Microbiology, 241:318-324.

Boxman, I. L., L. Verhoef, H. Vennema, S. Ngui, I. H. Friesema, C. Whiteside, D. Lees, and M. Koopmans. (2016). International linkage of two food-borne hepatitis A clusters through traceback of mussels, the Netherlands, 2012. Eurosurveillance, 21(3), 30113-30121.

Campos, C. J., Avant, J., Gustar, N., Lowther, J., Powell, A., Stockley, L., & Lees, D. N. (2015). Fate of human noroviruses in shellfish and water impacted by frequent sewage pollution events. Environmental Science & Technology, 49(14), 8377-8385.

Desselberger, U., & Goodfellow, I. (2014). Noroviruses: a global cause of acute gastroenteritis. The Lancet Infectious Diseases, 14(8), 664-665.

Karst, S. M., Wobus, C. E., Goodfellow, I. G., Green, K. Y., & Virgin, H. W. (2014). Advances in norovirus biology. Cell Host & Microbe, 15(6), 668-680.

Karst, S. M., Zhu, S., & Goodfellow, I. G. (2015). The molecular pathology of noroviruses. The Journal of Pathology, 235(2), 206-216.

Alhatlani, B., Vashist, S., & Goodfellow, I. (2015). Functions of the 5′ and 3′ ends of calicivirus genomes. Virus Research, 206, 134-143.

Thorne, L. G., & Goodfellow, I. G. (2014). Norovirus gene expression and replication. Journal of General Virology, 95(2), 278-291.

Campos, C. J., & Lees, D. N. (2014). Environmental transmission of human noroviruses in shellfish waters. Applied and Environmental Microbiology, 80(12), 3552-3561.

Flannery, J., Rajko‐Nenow, P., Winterbourn, J. B., Malham, S. K., & Jones, D. L. (2014). Effectiveness of cooking to reduce Norovirus and infectious F‐specific RNA bacteriophage concentrations in Mytilus edulis. Journal of Applied Microbiology, 117(2), 564-571.

Winterbourn, J. B., Clements, K., Lowther, J., Malham, S. K., McDonald, J. E., Jones, D. L., … & Zammit, A. (2014). Evaluation of spatial contamination patterns for norovirus and faecal indicator bacteria near to a coastal sewage discharge using Mytilus edulis as biosentinels. In Proceedings of the 9th International Conference on Molluscan Shellfish Safety, Sydney, Australia, 17-22 March 2013. (pp. 89-92). Organising Committee, International Conference on Molluscan Shellfish Society.

Carlos JA, C., Justin, A., James, L., Dale, T., & Lees, D. N. (2013). Levels of norovirus and E. coli in untreated, biologically treated and UV-disinfected sewage effluent discharged to a shellfish water. Journal of Water Resource and Protection, 2013. DOI:10.4236/jwarp.2013.510101

Lowther, J. A., Gustar, N. E., Powell, A. L., Hartnell, R. E., & Lees, D. N. (2012). Two-year systematic study to assess norovirus contamination in oysters from commercial harvesting areas in the United Kingdom. Applied and Environmental Microbiology, 78(16), 5812-5817.

Hartnell, R., Lowther, J., Avant, J., Dancer, D., Lees, D., & Russell, J. (2012). The development of LENTICULES™ as reference materials for noroviruses. Journal of Applied Microbiology, 112(2), 338-345.

Lowther, J. A., Gustar, N. E., Hartnell, R. E., & Lees, D. N. (2012). Comparison of norovirus RNA levels in outbreak-related oysters with background environmental levels. Journal of Food Protection, 75(2), 389-393.

Bosch, A., Bidawid, S., Le Guyader, S., Lees, D., & Jaykus, L. A. (2011). Norovirus and Hepatitis A virus in shellfish, soft fruits and water.

Hoorfar, J., Schultz, A. C., Lees, D. N., Bosch, A., Jordan, K., Butler, F., & Prugger, R. (2011). Foodborne viruses: understanding the risks and developing rapid surveillance and control measures. Food chain integrity: a holistic approach to food traceability, safety, quality and authenticity, 88-104.

Lees, D., & CENWG TAG (2010). International standardisation of a method for detection of human pathogenic viruses in molluscan shellfish. Food and Environmental Virology, 2(3), 146-155.