Publications

Recent publications of project members regarding foodborne and waterborne enteric viruses and SARS-CoV-2:

Brunner, F. S., Brown, M. R., Bassano, I., Denise, H., Khalifa, M. S., Wade, M. J., van Aerle, R., Kevill, J. L., Jones, D. L., Farkas, K., Jeffries, A. R., Cairns, E., Wierzbicki, C., & Paterson, S. (2022). City-wide wastewater genomic surveillance through the successive emergence of SARS-COV-2 alpha and Delta variants. Water Research, 226, 119306. https://doi.org/10.1016/j.watres.2022.119306

Jones, D. L., Grimsley, J. M., Kevill, J. L., Williams, R., Pellett, C., Lambert-Slosarska, K., Singer, A. C., Williams, G. B., Bargiela, R., Brown, R. W., Wade, M. J., & Farkas, K. (2022). Critical evaluation of different passive sampler materials and approaches for the recovery of SARS-COV-2, faecal-indicator viruses and bacteria from wastewater. Water, 14(21), 3568. https://doi.org/10.3390/w14213568

Jones, D. L., Rhymes, J. M., Wade, M. J., Kevill, J. L., Malham, S. K., Grimsley, J. M. S., Rimmer, C., Weightman, A. J., & Farkas, K. (2023). Suitability of aircraft wastewater for Pathogen Detection and Public Health Surveillance. Science of The Total Environment, 856, 159162. https://doi.org/10.1016/j.scitotenv.2022.159162

Robins, K., Leonard, A. F., Farkas, K., Graham, D. W., Jones, D. L., Kasprzyk-Hordern, B., Bunce, J. T., Grimsley, J. M., Wade, M. J., Zealand, A. M., & McIntyre-Nolan, S. (2022). Research needs for optimising wastewater-based epidemiology monitoring for Public Health Protection. Journal of Water and Health, 20(9), 1284–1313. https://doi.org/10.2166/wh.2022.026

Farkas, K., Pellett, C., Alex-Sanders, N., Bridgman, M. T., Corbishley, A., Grimsley, J. M., Kasprzyk-Hordern, B., Kevill, J. L., Pântea, I., Richardson-O’Neill, I. S., Lambert-Slosarska, K., Woodhall, N., & Jones, D. L. (2022). Comparative assessment of filtration- and precipitation-based methods for the concentration of SARS-COV-2 and other viruses from wastewater. Microbiology Spectrum, 10(4). https://doi.org/10.1128/spectrum.01102-22

Kevill, J. L., Lambert-Slosarska, K., Pellett, C., Woodhall, N., Richardson-O’Neill, I., Pântea, I., Alex-Sanders, N., Farkas, K., & Jones, D. L. (2022). Assessment of two types of passive sampler for the efficient recovery of SARS-COV-2 and other viruses from wastewater. Science of The Total Environment, 838, 156580. https://doi.org/10.1016/j.scitotenv.2022.156580

Wastewater Monitoring in Wales, National COVID-19 Report (released every week). Example: Report Date: 17 March 2022. Welsh Government.

Tlhagale, M., Liphadzi, S., … Jones, D.L. (2022) Establishment of local wastewater-based surveillance programmes in response to the spread and infection of COVID-19-case studies from South Africa, the Netherlands, Turkey and England. Journal or Water and Health 20, 287–299. https://doi.org/10.2166/wh.2022.185

Adriaenssens, E. M., Farkas, K., McDonald, J. E., Jones, D. L., Allison, H. E., & McCarthy, A. J. (2021). Tracing the fate of wastewater viruses reveals catchment-scale virome diversity and connectivity. Water research203, 117568. https://doi.org/10.1016/j.watres.2021.117568

Fitzgerald, S. F., Rossi, G., Low, A. S., McAteer, S. P., O’Keefe, B., Findlay, D., Cameron, G. J., Pollard, P., Singleton, P., Ponton, G., Singer, A. C., Farkas, K., Jones, D., Graham, D. W., Quintela-Baluja, M., Tait-Burkard, C., Gally, D. L., Kao, R., & Corbishley, A. (2021). Site Specific Relationships between COVID-19 Cases and SARS-CoV-2 Viral Load in Wastewater Treatment Plant Influent. Environmental Science & Technology55(22), 15276–15286. https://doi.org/10.1021/acs.est.1c05029

Singer, A.C., Jones, D.L. (2022) Testing sewage has helped track COVID – soon it could reveal much more about the UK’s health. The Conversation 23-03-2022

Kevill, J., Pellett, C., Farkas, K., Brown, M., Bassano, I., Denise, H., McDonald, J., Malham, S., Porter, J., Warren, J., Evens, N., Paterson, S., Singer, A. and Jones, D. (2022) A comparison of precipitation and filtration-based SARS-CoV-2 recovery methods and the influence of temperature, turbidity, and surfactant load in urban wastewater. Science of The Total Environment, 808: 151916. https://doi.org/10.1016/j.scitotenv.2021.151916

Wade, M., Lo Jacomo, A., Armenise, E., Brown, M., Bunce, J., Cameron, G., Fang, Z., Farkas, K., Gilpin, D., Graham, D., Grimsley, J., Hart, A., Hoffmann, T., Jackson, K., Jones, D., Lilley, C., McGrath, J., McKinley, J., McSparron, C., Nejad, B., Morvan, M., Quintela-Baluja, M., Roberts, A., Singer, A., Souque, C., Speight, V., Sweetapple, C., Walker, D., Watts, G., Weightman, A. and Kasprzyk-Hordern, B. (2021) Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes. Journal of Hazardous Materials 424:127456. https://doi.org/10.1016/j.jhazmat.2021.127456

Dancer, S., Li, Y., Hart, A., Tang, J., Jones, D.L. (2021) What is the risk of acquiring SARS-CoV-2 from the use of public toilets? The Science of the Total Environment 792:148341. https://doi.org/10.1016/j.scitotenv.2021.148341

Farkas, K., Green, E., Rigby, D. Cross, P., Tyrrel, S., Malham, S.K., Jones, D.L. (2021) Investigating awareness, fear and control associated with norovirus and other pathogens and pollutants using best–worst scaling. Scientific Reports 11,11194. https://doi.org/10.1038/s41598-021-90704-7

Hillary, L. S., Farkas, K., Maher, K. H., Lucaci, A., Thorpe, J., Distaso, M. A., Gaze, W. H., Paterson, S., Burke, T., Connor, T. R., McDonald, J. E., Malham, S. K., & Jones, D. L. (2021). Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK. Water Research, 117214. https://doi.org/10.1016/j.watres.2021.117214

Bashawri, YM (2021) Impact of Sediment Concentration on the Survival of Wastewater-Derived bla(CTX-M-15)-Producing E. coli, and the Implications for Dispersal into Estuarine Waters. International Journal of Environmental Research and Public Health 17, 7608. https://doi.org/10.3390/ijerph17207608

Polo, D., et al. (2020) Making waves: Wastewater-based epidemiology for COVID-19-approaches and challenges for surveillance and prediction. Water Research 186, 116404. https://doi.org/10.1016/j.watres.2020.116404

Farkas, K., Hillary, LS., Thorpe, J., Walker, DI., Lowther, JA., McDonald, JE., Malham, SK, Jones DL  (2021) Concentration and Quantification of SARS-CoV-2 RNA in Wastewater Using Polyethylene Glycol-Based Concentration and qRT-PCR. Methods and Protocols 4, 17. https://doi.org/10.3390/mps4010017

Wade, M., et al. (2020) Wastewater COVID-19 monitoring in the UK: summary for SAGE – 19 November 2020. https://www.gov.uk/government/publications/defrajbc-wastewater-covid-19-monitoring-in-the-uk-summary-19-november-2020

Jones, D.L., Baluja, M.Q., Graham, D.W., et al. (2020) Shedding of SARS-CoV-2 in feces and urine and its potential role in person-to-person transmission and the environment-based spread of COVID-19. Science of the Total Environment 749, 141364. https://doi.org/10.1016/j.scitotenv.2020.141364 

Ahmed, W., Bertsch, P., Bivins, A., Bibby, K., Farkas, K., Gathercole, A., … & Mueller, J. (2020). Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater. Science of the Total Environment, 739, 139960. https://doi.org/10.1016/j.scitotenv.2020.139960

Farkas, K., Hillary, L. S., Malham, S. K., McDonald, J. E., & Jones, D. L. (2020). Wastewater and public health: the potential of wastewater surveillance for monitoring COVID-19. Current Opinion in Environmental Science & Health. 17, 14-20. https://doi.org/10.1016/j.coesh.2020.06.001

Bivins, A., North, D., Ahmad, A., Ahmed, W., Alm, E., Been, F., … & Buttiglieri, G. (2020). Wastewater-Based Epidemiology: Global Collaborative to Maximize Contributions in the Fight Against COVID-19. Environmental Science and Technology, https://doi.org/10.1021/acs.est.0c02388

Farkas, K., Walker, D. I., Adriaenssens, E. M., McDonald, J. E., Hillary, L. S., Malham, S. K., & Jones, D. L. (2020). Viral indicators for tracking domestic wastewater contamination in the aquatic environment. Water Research, 181, 115926. https://doi.org/10.1016/j.watres.2020.115926

Farkas, K., Mannion, F., Hillary, L. S., Malham, S. K., & Walker, D. I. (2020). Emerging technologies for the rapid detection of enteric viruses in the aquatic environment. Current Opinion in Environmental Science & Health. 16, 1-6. https://doi.org/10.1016/j.coesh.2020.01.007

Robins, P. E., Farkas, K., Cooper, D., Malham, S. K., & Jones, D. L. (2019). Viral dispersal in the coastal zone: A method to quantify water quality risk. Environment International, 126, 430-442. https://doi.org/10.1016/j.envint.2019.02.042

Farkas, K., Adriaenssens, E.M., Walker, D.I. et al. (2019) Critical Evaluation of CrAssphage as a Molecular Marker for Human-Derived Wastewater Contamination in the Aquatic Environment. Food and Environmental Virology. 11, 113-119. https://doi.org/10.1007/s12560-019-09369-1

Lowther, J. A., Cross, L., Stapleton, T., Gustar, N. E., Walker, D. I., Sills, M., … & Lees, D. N. (2019). Use of F-Specific RNA Bacteriophage to Estimate Infectious Norovirus Levels in Oysters. Food and Environmental Virology, 11(3), 247-258. https://doi.org/10.1007/s12560-019-09383-3

Walker, D. I., Cross, L. J., Stapleton, T. A., Jenkins, C. L., Lees, D. N., & Lowther, J. A. (2019). Assessment of the Applicability of Capsid-Integrity Assays for Detecting Infectious Norovirus Inactivated by Heat or UV Irradiation. Food and environmental virology, 11(3), 229-237. https://doi.org/10.1007/s12560-019-09390-4

Farkas, K., Marshall, M., Cooper, D., McDonald, J., Malham, S., Peters, D., Maloney, J., Jones, D. (2018) Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric viruses. Environmental Science and Pollution Researchhttps://doi.org/10.1007/s11356-018-3261-y

Farkas, K., McDonald, J.E., Malham, S.K., Jones, D.L. (2018) Two-Step Concentration of Complex Water Samples for the Detection of Viruses. Methods and Protocols. 1, 35. https://doi.org/10.3390/mps1030035

Adriaenssens, E., Farkas, K., Harrison, C., Jones, D.L., Allison, H.E., McCarthy, A.J. (2018) Viromic analysis of wastewater input to a river catchment reveals a diverse assemblage of RNA viruses. mSystems. https://doi.org/10.1128/mSystems.00025-18

Farkas, K., Cooper, D.M., McDonald, J.E., Malham, S. K., de Rougemont, A., Jones, D. (2018) Seasonal and spatial dynamics of enteric viruses in wastewater and in riverine and estuarine receiving waters. Science of the Total Environment, 634:1174-1183 https://doi.org/10.1016/j.scitotenv.2018.04.038

Farkas, K., Peters, D.E., McDonald, J.E., de Rougemont, A., Malham, S. K., Jones, D. (2017) Evaluation of Two Triplex One-Step qRT-PCR Assays for the Quantification of Human Enteric Viruses in Environmental Samples. Food and Environmental Virology, 9(3):342–349. http://link.springer.com/article/10.1007/s12560-017-9293-5

Hassard, F., Sharp, J. H., Taft, H., Le Vay, L., Harris, J. P., McDonald, J. E., Tuson, K., Wilson, J., Jones, D., Malham, S. (2017) Critical Review on the Public Health Impact of Norovirus Contamination in Shellfish and the Environment: A UK Perspective. Food and Environmental Virology, 9(2):123–141. http://dx.doi.org/10.1007/s12560-017-9279-3

Farkas, K., Hassard, F., McDonald, J. E., Malham, S. K. and Jones, D. (2016) Evaluation of molecular methods for the detection and quantification of pathogen-derived nucleic acids in sediment.  Frontiers in Microbiology. http://dx.doi.org/10.3389/fmicb.2017.00053

Hassard, F., Gwyther, C. L.,  Farkas, K.,  Andrews, A.,  Jones, V., Cox, B.,  Brett, H., Jones, D.,  McDonald, J. E., and  Malham, S. K.  (2016)   Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review.  Frontiers in Microbiology. http://dx.doi.org/10.3389/fmicb.2016.01692

Campos, C. J., Kershaw, S., Morgan, O.C. & Lees, D. N. (2016). Risk factors for norovirus contamination of shellfish water catchments in England and Wales. International Journal of Food Microbiology, 241:318-324. http://dx.doi.org/10.1016/j.ijfoodmicro.2016.10.028

Boxman, I. L., L. Verhoef, H. Vennema, S. Ngui, I. H. Friesema, C. Whiteside, D. Lees, and M. Koopmans. (2016). International linkage of two food-borne hepatitis A clusters through traceback of mussels, the Netherlands, 2012. Eurosurveillance, 21(3), 30113-30121.

Campos, C. J., Avant, J., Gustar, N., Lowther, J., Powell, A., Stockley, L., & Lees, D. N. (2015). Fate of human noroviruses in shellfish and water impacted by frequent sewage pollution events. Environmental Science & Technology, 49(14), 8377-8385.

Desselberger, U., & Goodfellow, I. (2014). Noroviruses: a global cause of acute gastroenteritis. The Lancet Infectious Diseases, 14(8), 664-665.

Karst, S. M., Wobus, C. E., Goodfellow, I. G., Green, K. Y., & Virgin, H. W. (2014). Advances in norovirus biology. Cell Host & Microbe, 15(6), 668-680.

Karst, S. M., Zhu, S., & Goodfellow, I. G. (2015). The molecular pathology of noroviruses. The Journal of Pathology, 235(2), 206-216.

Alhatlani, B., Vashist, S., & Goodfellow, I. (2015). Functions of the 5′ and 3′ ends of calicivirus genomes. Virus Research, 206, 134-143.

Thorne, L. G., & Goodfellow, I. G. (2014). Norovirus gene expression and replication. Journal of General Virology, 95(2), 278-291.

Campos, C. J., & Lees, D. N. (2014). Environmental transmission of human noroviruses in shellfish waters. Applied and Environmental Microbiology, 80(12), 3552-3561.

Flannery, J., Rajko‐Nenow, P., Winterbourn, J. B., Malham, S. K., & Jones, D. L. (2014). Effectiveness of cooking to reduce Norovirus and infectious F‐specific RNA bacteriophage concentrations in Mytilus edulis. Journal of Applied Microbiology, 117(2), 564-571.

Winterbourn, J. B., Clements, K., Lowther, J., Malham, S. K., McDonald, J. E., Jones, D. L., … & Zammit, A. (2014). Evaluation of spatial contamination patterns for norovirus and faecal indicator bacteria near to a coastal sewage discharge using Mytilus edulis as biosentinels. In Proceedings of the 9th International Conference on Molluscan Shellfish Safety, Sydney, Australia, 17-22 March 2013. (pp. 89-92). Organising Committee, International Conference on Molluscan Shellfish Society.

Carlos JA, C., Justin, A., James, L., Dale, T., & Lees, D. N. (2013). Levels of norovirus and E. coli in untreated, biologically treated and UV-disinfected sewage effluent discharged to a shellfish water. Journal of Water Resource and Protection, 2013. DOI:10.4236/jwarp.2013.510101

Lowther, J. A., Gustar, N. E., Powell, A. L., Hartnell, R. E., & Lees, D. N. (2012). Two-year systematic study to assess norovirus contamination in oysters from commercial harvesting areas in the United Kingdom. Applied and Environmental Microbiology, 78(16), 5812-5817.

Hartnell, R., Lowther, J., Avant, J., Dancer, D., Lees, D., & Russell, J. (2012). The development of LENTICULES™ as reference materials for noroviruses. Journal of Applied Microbiology, 112(2), 338-345.

Lowther, J. A., Gustar, N. E., Hartnell, R. E., & Lees, D. N. (2012). Comparison of norovirus RNA levels in outbreak-related oysters with background environmental levels. Journal of Food Protection, 75(2), 389-393.

Bosch, A., Bidawid, S., Le Guyader, S., Lees, D., & Jaykus, L. A. (2011). Norovirus and Hepatitis A virus in shellfish, soft fruits and water.

Hoorfar, J., Schultz, A. C., Lees, D. N., Bosch, A., Jordan, K., Butler, F., & Prugger, R. (2011). Foodborne viruses: understanding the risks and developing rapid surveillance and control measures. Food chain integrity: a holistic approach to food traceability, safety, quality and authenticity, 88-104.

Lees, D., & CENWG TAG (2010). International standardisation of a method for detection of human pathogenic viruses in molluscan shellfish. Food and Environmental Virology, 2(3), 146-155.